Virginia Eshelman Johnson (born February 11, 1925 in Springfield, Missouri) was an American psychologist, best known as the junior member of the Masters and Johnson sexuality research team. Along with William Masters, she pioneered research into the nature of human sexual response and the diagnosis and treatment of sexual disorders and dysfunctions from 1957 until the 1990s.

Johnson met Masters in 1957 when he hired her as a research assistant to undertake a comprehensive study of human sexuality. They married in 1969, but divorced three decades later, largely bringing their joint research to an end.

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