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Individual differences |
Methods | Statistics | Clinical | Educational | Industrial | Professional items | World psychology |
Biological: Behavioural genetics · Evolutionary psychology · Neuroanatomy · Neurochemistry · Neuroendocrinology · Neuroscience · Psychoneuroimmunology · Physiological Psychology · Psychopharmacology (Index, Outline)
Photostimulation is the use of light to artificially activate biological compounds, cells, or even whole organisms. Photostimulation can be used to noninvasively probe the causal relationships between different biological processes, using only light. In the long run, photostimulation may be useful as a therapy, using light to adjust the biological state of human patients.
Photostimulation methods fall into two general categories: one set of methods uses light to uncage a compound that then becomes biochemically active, binding to a downstream effector. For example,uncaging glutamate is useful for finding excitatory connections between neurons, since the uncaged glutamate mimics the natural synaptic activity of one neuron impinging upon another. The other major photostimulation method is the use of light to activate a light-sensitive protein such as rhodopsin, which can then excite the cell expressing the opsin. It has been shown that channelrhodopsin-2, a monolithic protein containing a light sensor and an ion channel, could provide stimulation of appropriate speed and magnitude.
See glutamate uncaging tutorial at Sherman Lab Laser Uncaging
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