Phenylalanine hydroxylase (EC 126.96.36.199) is an enzyme which catalyses the reaction causing the addition of an hydroxyl group to the end of the 6-carbon aromatic ring of phenylalanine, such that it becomes tyrosine. Phenylalanine hydroxylase is the rate-limiting enzyme of the metabolic pathway which degrades excess phenylalanine. Mutations in phenylalanine hydroxylase which result in lower activity are the cause of the disease phenylketonuria, or PKU. The other substrates in the reaction are molecular oxygen and tetrahydrobiopterin. Tetrahydrobiopterin is a member of the group of redox biochemicals known as pteridines. PAH is the gene that encodes for phenylalanine hydroxylase.
Phenylalanine hydroxylase is closely related to two other enzymes, tryptophan hydroxylase (EC number 188.8.131.52), which controls levels of serotonin in the brain and the gastrointestinal tract, and tyrosine hydroxylase (EC number 184.108.40.206), which controls levels of dopamine, epinephrine, and norepinephrine in the brain and the adrenal medulla. The three enzymes are homologous, that is, are thought to have evolved from the same ancient hydroxylase.
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Phenylalanine hydroxylase is a tetramer composed of two dimers, that is, composed of two complexes of two subunits each. Each subunit is in turn composed of three domains, a regulatory domain, a catalytic domain, and a tetramerization domain. The regulatory domain is composed of the approximately 115 amino acids nearest the amino terminal of the subunit. The catalytic domain is composed of the next approximately 300 amino acids, and is responsible for all of the catalytic activity of the enzyme. The tetramerization domain consists of the remaining amino acids and through the formation of a "coiled-coil" arrangement of amino acids, holds the tetrameric structure of the holoenzyme together with a "leucine zipper". Phenylalanine hydroxylase contains one bound iron atom per subunit which is necessary for catalytic activity.de:Phenylalaninhydroxylase
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