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Porosomes or fusion pores are cup-shaped structures in the cell membranes of eukaryotic cells where vesicles dock in the process of vesicle fusion and secretion. The porosomes are few nanometers in size and contain many different types of protein, especially chloride and calcium channels, actin, and SNARE proteins that mediate the docking and fusion of the vesicles with the cell membrane. Once the vesicles have docked with the SNARE proteins, they swell, which increases their internal pressure. They then transiently fuse at the base of the porosome, and these pressurized contents are ejected from the cell. Examination of cells following secretion using electron microscopy, demonstrate increased presence of partially empty vesicles following secretion. This suggested that during the secretory process, only a portion of the vesicular contents are able to exit the cell. This could only be possible if the vesicle were to temporarily establish continuity with the cell plasma membrane, expel a portion of its contents, then detach, reseal, and withdraw into the cytosol (endocytose). In this way, the secretory vesicle could be reused for subsequent rounds of exo-endocytosis, until completely empty of its contents.
Porosomes vary in size depending on the cell type. Porosome in the exocrine pancreas and in endocrine and neuroendocrine cells range from 100 nm to 180 nm in diameter while in neurons they range from 10 nm to 15 nm (about 1/10 the size of pancreatic porosomes). When a secretory vesicle containing v-SNARE docks at the porosome base containing t-SNARE, membrane continuity (ring complex) is formed between the two. The size of the t/v-SNARE complex is directly proportional to the size of the vesicle. These vesicles contain dehydrated proteins (non-active) which are activated once they are hydrated. GTP is required for the transport of water through the water channels or Aquaporins, and ions through ion channels to hydrate the vesicle. Once the vesicle fuses at the porosome base, the contents of the vesicle at high pressure are ejected from the cell.
Generally the pososomes are opened and closed by actin, but neurons require a fast response therefore they have central plugs that open to release contents and close to stop the release (the composition of the central plug is yet to be discovered). Porosomes have been demonstrated to be the universal secretory machinery in cells.
History of discoveryEdit
The porosome was discovered in the early to mid-1990s by a team led by Professor Bhanu Pratap Jena at Yale University School of Medicine, using atomic force microscopy. Dr. Bhanu Jena is the "Distinguished Professor of Physiology" at the Wayne State University School of Medicine.
- ↑ 1.0 1.1 Anderson LL (2006). Discovery of the 'porosome'; the universal secretory machinery in cells. J. Cell. Mol. Med. 10 (1): 126–31.
- ↑ Jena BP (2003). Fusion pore or porosome: structure and dynamics. J. Endocrinol. 176 (2): 169–74.
- ↑ Jena BP (2004). Discovery of the Porosome: revealing the molecular mechanism of secretion and membrane fusion in cells. J. Cell. Mol. Med. 8 (1): 1–21.
- ↑ Jena, B.P. (2012), NanoCellBiology of Secretion. Imaging its cellular and molecular underpinnings, Springer Briefs in Biological Imaging, pp. 1–70, ISBN 978-1-4614-2437-6
- ↑ http://joe.endocrinology-journals.org/cgi/reprint/176/2/169.pdf
- ↑ http://www.med.wayne.edu/physiology/facultyprofile/jena/pdf%20files/jena%20fusion%20pore.pdf
- ↑ Boron, WF & Boulpaep, EL (2012), Medical Physiology. A Cellular and Molecular Approach, Philadelphia: Elsevier, http://www.studentconsult.com/content/9781437717532/boron/Webnote8.htm#fig1
- Molecular Machinery & Mechanism of Cell Secretion Jena Lab at Wayne State University School of Medicine
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