Barnes maze

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The Barnes maze is a psychological laboratory experiment used to measure spatial learning and memory. The test subjects are usually rodents such as mice or lab rats, which either serve as a control or may have some genetic variable or deficiency present in them which will cause them to react differently to the maze.

Set-Up

The Barnes maze consists of a circular table with 20 circular holes around the circumference of the table. Under each hole is a slot for a box, called the drop box. The goal of the maze is to reach the drop box, which is a box that has an open top, and can be reached through one of the holes in the top of the table. Anything on the surface of the table itself is subject to high winds and bright lights. These serve as negative reinforcement, motivating the test subject to seek shelter. The only shelter available is the drop box, to which the test subject must flee. In order to accustom the test subject to the maze, it is guided into the drop box by a sheltering hand. After four to five runs, a normal test subject can quickly make a beeline for the drop hole.

Problems

One problem with the Barnes maze is the constant need to clean up after each trial. When nervous, most rodents will urinate and defecate on the table, necessitating the experimenter to clean up the entire table with disinfectant. Also, other animals who have not yet been tested cannot be allowed to witness the experiment in action, or they will have prior knowledge to the nature of the experiment, which will allow them to find the drop box more easily^{[How to reference and link to summary or text]}. Another consequence of untested animals witnessing the experiment is pre-experiment nervousness, which will make the animals experience more stress, and will make it harder for the experimenter to take hold of the animal.